"Ssdna" Example Sentences
1. The ssDNA was denatured and hybridized to a complementary sequence.
2. The ssDNA was amplified using PCR.
3. The ssDNA was sequenced using next-generation sequencing technology.
4. The ssDNA was labeled with fluorescent dye for visualization.
5. The ssDNA was inserted into a cloning vector for transformation.
6. The ssDNA was purified using gel electrophoresis.
7. The ssDNA was extracted from a bacterial culture.
8. The ssDNA was hybridized to a microarray for gene expression analysis.
9. The ssDNA was synthesized using an oligonucleotide synthesizer.
10. The ssDNA was used as a template for in vitro transcription.
11. The ssDNA was crosslinked to a protein for protein-DNA binding studies.
12. The ssDNA was annealed to a primer for DNA sequencing.
13. The ssDNA was attached to a solid support for DNA microarray analysis.
14. The ssDNA was labeled with biotin for affinity purification.
15. The ssDNA was used as a probe for hybridization to a target DNA sequence.
16. The ssDNA was treated with restriction enzymes for cloning purposes.
17. The ssDNA was circularized for use as a plasmid in bacterial transformation.
18. The ssDNA was mutated using site-directed mutagenesis.
19. The ssDNA was modified with a chemical crosslinker for structural analysis.
20. The ssDNA was incorporated into a TIRF microscopy imaging system.
21. The ssDNA was used as a substrate for DNA polymerase activity.
22. The ssDNA was ligated to another piece of DNA for gene fusion construction.
23. The ssDNA was modified with methyl groups to study DNA methylation.
24. The ssDNA was amplified using reverse transcription PCR from RNA.
25. The ssDNA was used as a model system for DNA-protein interaction studies.
26. The ssDNA was produced using rolling circle amplification.
27. The ssDNA was labeled with radioisotopes for radioligand binding assays.
28. The ssDNA was used as a control in a DNA footprinting experiment.
29. The ssDNA was treated with sodium bisulfite for DNA methylation analysis.
30. The ssDNA was hybridized to a nanopore for single-molecule sequencing.
Common Phases
1. Single-stranded DNA can be easily denatured to form
ssDNA.
2. The use of PCR amplification requires an initial step of denaturing dsDNA to
ssDNA.
3.
ssDNA can be a useful probe in hybridization studies to detect complementary sequences.
4. RNA viruses replicate using ssRNA as a template to produce
ssDNA intermediates.
5. A variety of techniques, such as PCR, gel electrophoresis, and Southern blotting, can be used to detect and analyze
ssDNA.